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Doxorubicin–transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells

dc.contributor.authorWigner, Paulina
dc.contributor.authorZielinski, Krzysztof
dc.contributor.authorŁabieniec-Watała, Magdalena
dc.contributor.authorMarczak, Agnieszka
dc.contributor.authorSzwed, Marzena
dc.date.accessioned2021-12-16T11:58:43Z
dc.date.available2021-12-16T11:58:43Z
dc.date.issued2021
dc.identifier.citationWigner, P., Zielinski, K., Labieniec-Watala, M. et al. Doxorubicin–transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells. Sci Rep 11, 4544 (2021). https://doi.org/10.1038/s41598-021-84146-4pl_PL
dc.identifier.urihttp://hdl.handle.net/11089/40077
dc.description.abstractDoxorubicin (DOX) is considered one of the most powerful chemotherapeutic agents but its clinical use has several limitations, including cardiomyopathy and cellular resistance to the drug. By using transferrin (Tf) as a drug carrier, however, the adverse effects of doxorubicin as well as drug resistance can be reduced. The main objective of this study was to determine the exact nature and extent to which mitochondrial function is influenced by DOX–Tf conjugate treatment, specifically in human breast adenocarcinoma cells. We assessed the potential of DOX–Tf conjugate as a drug delivery system, monitoring its cytotoxicity using the MTT assay and ATP measurements. Moreover, we measured the alterations of mitochondrial function and oxidative stress markers. The effect of DOX–Tf was the most pronounced in MDA-MB-231, triple-negative breast cancer cells, whereas non-cancer endothelial HUVEC-ST cells were more resistant to DOX–Tf conjugate than to free DOX treatment. A different sensitivity of two investigate breast cancer cell lines corresponded to the functionality of their cellular antioxidant systems and expression of estrogen receptors. Our data also revealed that conjugate treatment mediated free radical generation and altered the mitochondrial bioenergetics in breast cancer cells.pl_PL
dc.description.sponsorshipThis work was supported in part by Grant No. B1511000001026.02 of the University of Lodz, Poland.pl_PL
dc.language.isoenpl_PL
dc.publisherSpringer Naturepl_PL
dc.relation.ispartofseriesScientific Reports;11
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbiochemistrypl_PL
dc.subjectbiophysicspl_PL
dc.subjectbiotechnologypl_PL
dc.subjectcancerpl_PL
dc.subjectcell biologypl_PL
dc.subjectchemical biologypl_PL
dc.subjectdrug discoverypl_PL
dc.subjectmolecular biologypl_PL
dc.titleDoxorubicin–transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cellspl_PL
dc.typeArticlepl_PL
dc.page.number14pl_PL
dc.contributor.authorAffiliationDepartment of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Polandpl_PL
dc.identifier.eissn2045-2322
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dc.identifier.doi10.1038/s41598-021-84146-4
dc.relation.volume4544pl_PL
dc.disciplinenauki biologicznepl_PL


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