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Phosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexes

dc.contributor.authorFerenc, Małgorzata
dc.contributor.authorPędziwiatr-Werbicka, Elżbieta
dc.contributor.authorKlajnert-Maculewicz, Barbara
dc.contributor.authorBryszewska, Maria
dc.contributor.authorNowak, Katarzyna
dc.contributor.authorMajoral, Jean-Pierre
dc.date.accessioned2021-11-17T10:37:25Z
dc.date.available2021-11-17T10:37:25Z
dc.date.issued2013
dc.identifier.citationFerenc, M.; Pedziwiatr-Werbicka, E.; Nowak, K.E.; Klajnert, B.; Majoral, J.-P.; Bryszewska, M. Phosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexes. Molecules 2013, 18, 4451-4466. https://doi.org/10.3390/molecules18044451pl_PL
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/11089/39788
dc.description.abstractThere are many types of dendrimers used as nanomolecules for gene delivery but there is still an ongoing search for ones that are able to effectively deliver drugs to cells. The possibility of gene silencing using siRNA gives hope for effective treatment of numerous diseases. The aim of this work was to investigate in vitro biophysical properties of dendriplexes formed by siRNA and cationic phosphorus dendrimers of 3rd and 4th generation. First, using the ethidium bromide intercalation method, it was examined whether dendrimers have an ability to form complexes with siRNA. Next, the characterisation of dendriplexes formed at different molar ratios was carried out using biophysical methods. The effects of zeta potential, size and changes of siRNA conformation on the complexation with dendrimers were examined. It was found that both phosphorus dendrimers interacted with siRNA. The zeta potential values of dendriplexes ranged from negative to positive and the hydrodynamic diameter depended on the number of dendrimer molecules in the complex. Furthermore, using circular dichroism spectroscopy it was found that cationic phosphorus dendrimers changed only slightly the shape of siRNA CD spectra, thus they did not induce significant changes in the nucleic acid secondary structure during complex formation.pl_PL
dc.description.sponsorshipStudies were funded by the project ―Biological properties and biomedical applications of dendrimers‖ operated within the Foundation for Polish Science Team Programme co-financed by the EU European Regional Development Fund. This work was also supported by the COST TD0802 project and by the CNRS, France.pl_PL
dc.language.isoenpl_PL
dc.publisherMDPIpl_PL
dc.relation.ispartofseriesMolecules;18
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectsiRNApl_PL
dc.subjectphosphorus dendrimerspl_PL
dc.subjectchronic myeloid leukemiapl_PL
dc.subjectgene therapypl_PL
dc.subjectdendriplexpl_PL
dc.titlePhosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexespl_PL
dc.typeArticlepl_PL
dc.page.number4451-4466pl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDivision of Radiobiology, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationLaboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse Cedex 4, Francepl_PL
dc.referencesVerma, M.; Somia, N. Gene therapy—promises, problems and prospects. Nature 1997, 389, 239–242.pl_PL
dc.referencesHuang, L.; Viroonchatapan, E. Introduction. In Non-viral Vector for Gene Therapy; Huang, L., Wagner, M., Eds.; Academic Press: New York, NY, USA, 1999; pp. 3–22.pl_PL
dc.referencesHannon, G.J. RNA interference. Nature 2002, 418, 244–251.pl_PL
dc.referencesElbashir, S.M.; Harborth, J.; Lendeckel, W.; Yalcin, A.; Weber, K.; Tuschl, T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001, 411, 494–498.pl_PL
dc.referencesTomalia, D.A.; Baker, H.; Dewald, J.; Hall, M.; Kallos, G.; Martin, S.; Roeck, J.; Ryder, J.; Smith, P. A new class of polymers: Starburst-dendritic macromolecules. Polym. J. 1985, 17, 117–132.pl_PL
dc.referencesKlajnert, B.; Bryszewska, M. Dendrimers: Properties and applications. Acta Biochim. Pol. 2001, 48, 199–208.pl_PL
dc.referencesGalliot, C.; Larré, C.; Caminade, A.M.; Majoral, J.P. Regioselective stepwise growth of dendrimer units in the internal voids of a main dendrimer. Science 1997, 277, 1981–1984.pl_PL
dc.referencesMerino, S.; Brauge, L.; Caminade, A.M.; Majoral, J.P.; Taton, D.; Gnanou, Y. Synthesis and characterization of linear, Hyperbranched, and dendrimer-like polymers constituted of the same repeating unit. Chemistry 2001, 7, 3095–3105.pl_PL
dc.referencesCaminade, A.M.; Majoral, J.P. Water-soluble phosphorus-containing dendrimers. Prog. Polym. Sci. 2005, 30, 491–505pl_PL
dc.referencesSolassol, J.; Crozet, C.; Perrier, V.; Leclaire, J.; Béranger, F.; Caminade, A.M.; Meunier, B.; Dormont, D.; Majoral, J.P.; Lehmann, S. Cationic phosphorus-containing dendrimers reduce prion replication both in cell culture and in mice infected with scrapie. J. Gen. Virol. 2004, 85, 1791–1799.pl_PL
dc.referencesLoup, C.; Zanta, M.A.; Caminade, A.M.; Majoral, J.P.; Meunier, B. Preparation of water-soluble cationic phosphorus-containing dendrimers as DNA transfecting agents. Chem. Eur. J. 1999, 5, 3644–3650.pl_PL
dc.referencesMaksimenko, A.V.; Mandrouguine, V.; Gottikh, M.B.; Bertrand, J.R.; Majoral, J.P.; Malvy, C. Optimisation of dendrimer-mediated gene transfer by anionic oligomers. J. Gene. Med. 2003, 5, 61–71.pl_PL
dc.referencesIonov, M.; Wróbel, D.; Gardikis, K.; Hatziantoniou, S.; Demetzos, C.; Majoral, J.P.; Klajnert, B.; Bryszewska, M. Effect of phosphorus dendrimers on DMPC lipid membranes. Chem. Phys. Lipids 2011, 165, 408–413.pl_PL
dc.referencesWrobel, D.; Ionov, M.; Gardikis, K.; Demetzos, C.; Majoral, J.P.; Palecz, B.; Klajnert, B.; Bryszewska, M. Interactions of phosphorus-containing dendrimers with liposomes. Biochim. Biophys. Acta 2010, 1811, 221–226.pl_PL
dc.referencesGriffe, L.; Poupot, M.; Marchand, P.; Maraval, A.; Turrin, C.O.; Rolland, O.; Métivier, P.; Bacquet, G.; Fournié, J.J.; Caminade, A.M.; et al. Multiplication of human natural killer cells by nanosized phosphonate-capped dendrimers. Angew. Chem. Int. Ed. Engl. 2007, 46, 2523–2526.pl_PL
dc.referencesPoupot, M.; Griffe, L.; Marchand, P.; Maraval, A.; Rolland, O.; Martinet, L.; L'Faqihi-Olive, F.E.; Turrin, C.O.; Caminade, A.M.; Fournié, J.J.; et al. Design of phosphorylated dendritic architectures to promote human monocyte activation. FASEB J. 2006, 20, 2339–2351.pl_PL
dc.referencesHayder, M.; Poupot, M.; Baron, M.; Nigon, D.; Turrin, C.O.; Caminade, A.M.; Majoral, J.P.; Eisenberg, R.A.; Fournie, J.J.; Cantagrel, A.; et al. A phosphorus-based dendrimer targets inflammation and osteoclastogenesis in experimental arthritis. Sci. Transl. Med. 2011, 3, 81ra35.pl_PL
dc.referencesRolland, O.; Griffe, L.; Poupot, M.; Maraval, A.; Ouali, A.; Coppel, Y.; Fournié, J.J.; Bacquet, G.; Turrin, C.O.; Caminade, A.M.; et al. Tailored control and optimisation of the number of phosphonic acid termini on phosphorus-containing dendrimers for the ex vivo activation of human monocytes. Chemistry 2008, 14, 4836–4850.pl_PL
dc.referencesShcharbin, D.; Dzmitruk, V.; Shakhbazau, A.; Goncharova, N.; Seviaryn, I.; Kosmacheva, S.; Potapnev, M.; Pedziwiatr-Werbicka, E.; Bryszewska, M.; Talabaev, M.; et al. Fourth generation phosphorus-containing dendrimers: Prospective drug and gene delivery carrier. Pharmaceutics 2011, 3, 458–473.pl_PL
dc.referencesEftink, M.R. Fluorescence quenching reactions: Probing biological macromolecular structures. In Biophysical and Biochemical Aspects of Fluorescence Spectroscopy; Dewey, T.G., Ed.; Plenum: New York, NY, USA, 1991; pp. 1–41.pl_PL
dc.referencesAkhtar, S.; Hughes, M.D.; Khan, A.; Bibby, M.; Hussain, M.; Nawaz, Q.; Double, J.; Sayyed, P. The delivery of antisense therapeutics. Adv. Drug Deliver. Rev. 2000, 44, 3–21.pl_PL
dc.referencesAkhtar, S.; Juliano, R.L. Cellular and intracellular fate of antisense oligonucleotides. Trends Cell Biol. 1992, 2, 139–144.pl_PL
dc.referencesHussain, M.; Shchepinov, M.; Sohail, M.; Benter, I.F.; Hollins, A.J.; Southern, E.M.; Akhtar, S. A novel anionic dendrimer for improved cellular delivery of antisense oligonucleotides. J. Control. Release 2004, 99, 139–155.pl_PL
dc.referencesBumcrot, D.; Manoharan, M.; Koteliansky, V.; Sah, D.W. RNAi therapeutics: A potential new class of pharmaceutical drugs. Nat. Chem. Biol. 2006, 2, 711–719.pl_PL
dc.referencesKathlen, F.; Zon, G.; Rati, A.; Zhou, Q.; Yo, W. Targeting Nanoimmunoliposome Complex for Short Interfering RNA Delivery. Hum. Gene Ther. 2006, 17, 117–124.pl_PL
dc.referencesLam, J.K.W.; Liang, W.; Chan, H.K. Pulmonary delivery of therapeutic siRNA. Adv. Drug Deliver. Rev. 2011, 64, 1–15.pl_PL
dc.referencesJuliano, R.; Alam, M.R.; Dixit, V.; Kang, H. Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides. Nucleic Acids Res. 2008, 36, 4158–4171.pl_PL
dc.referencesAigner, A. Nonviral in vivo delivery of therapeutic small interfering RNAs. Curr. Opin. Mol. Ther. 2007, 9, 345–352.pl_PL
dc.referencesD’Emanuele, A.; Attwood, D. Dendrimer-drug interactions. Adv. Drug Deliver. Rev. 2005, 57, 2147–2162.pl_PL
dc.referencesQuillardet, P.; Hofnung, M. Ethidium bromide and safety—Readers suggest alternative solutions. Trends Genet. 1988, 4, 89–93.pl_PL
dc.referencesHashizume, H.; Imahori, M. Circular dichroism and the conformation of natural and synthetic polynucleotides. J. Biochem. 1967, 61, 738–749.pl_PL
dc.referencesTritton, T.R.; Crothers, D.M. Physical characterization of a ribosomal nucleoprotein complex. Biochemistry 1976, 15, 4377–4385.pl_PL
dc.referencesChiu, Y.L.; Rana, T.M. RNAi in human cells: Basic structural and functional features of small interfering RNA. Mol. Cell 2002, 10, 549–561.pl_PL
dc.referencesPlank, C.; Mechtler, K.; Szoka, F.C.; Wagner, E. Activation of the complement system by synthetic DNA complexes: A potential barrier to intravenous gene delivery. Hum. Gene Ther. 1996, 7, 1437–1446.pl_PL
dc.referencesPouton, C.W.; Seymour, L.W. Key issues in non-viral gene delivery. Adv. Drug Deliver. Rev. 1998, 34, 3–19pl_PL
dc.referencesJensen, L.B.; Pavan, G.M.; Kasimova, M.R.; Rutherford, S.; Danani, A.; Nielsen, H.M.; Foged, C. Elucidating the molecular mechanism of PAMAM-siRNA dendriplex self-assembly: Effect of dendrimer charge density. Int. J. Pharm. 2011, 416, 410–418.pl_PL
dc.referencesFang, M.; Cheng, Y.; Zhang, J.; Wu, Q.; Hu, J.; Zhao, L.; Xu, T. New insights into interactions between dendrimers and surfactants. 4. Fast-exchange/slow-exchange transitions in the structure of dendrimer-surfactant aggregates. J. Phys. Chem. B 2010, 114, 6048–6055.pl_PL
dc.referencesOh, Y.K.; Park, T.G. siRNA delivery systems for cancer treatment. Adv. Drug Deliver. Rev. 2009, 61, 850–862pl_PL
dc.referencesTsai, C.C.; Jain, S.C.; Sobel, H.M. Visualization of drug–nucleic acid interactions at atomic resolution. I. Structure of an ethidium/dinucleoside monophosphate crystalline complex, ethidium:5-iodouridylyl (3'–5') adenosine. J. Mol. Biol. 1977, 114, 301–315.pl_PL
dc.referencesPeng, Y.; Zhang, Q.; Nagasawa, H.; Okayasu, R.; Liber, H.; Bedford, J. Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human cells for radiation-induced chromosome damage, cell killing, and mutation. Cancer Res. 2002, 62, 6400–6404.pl_PL
dc.identifier.doi10.3390/molecules18044451
dc.relation.volume4pl_PL
dc.disciplinenauki biologicznepl_PL


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