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Phosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexes

dc.contributor.authorFerenc, Małgorzata
dc.contributor.authorPędziwiatr-Werbicka, Elżbieta
dc.contributor.authorKlajnert-Maculewicz, Barbara
dc.contributor.authorBryszewska, Maria
dc.contributor.authorNowak, Katarzyna
dc.contributor.authorMajoral, Jean-Pierre
dc.date.accessioned2021-11-17T10:37:25Z
dc.date.available2021-11-17T10:37:25Z
dc.date.issued2013
dc.identifier.citationFerenc, M.; Pedziwiatr-Werbicka, E.; Nowak, K.E.; Klajnert, B.; Majoral, J.-P.; Bryszewska, M. Phosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexes. Molecules 2013, 18, 4451-4466. https://doi.org/10.3390/molecules18044451pl_PL
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/11089/39788
dc.description.abstractThere are many types of dendrimers used as nanomolecules for gene delivery but there is still an ongoing search for ones that are able to effectively deliver drugs to cells. The possibility of gene silencing using siRNA gives hope for effective treatment of numerous diseases. The aim of this work was to investigate in vitro biophysical properties of dendriplexes formed by siRNA and cationic phosphorus dendrimers of 3rd and 4th generation. First, using the ethidium bromide intercalation method, it was examined whether dendrimers have an ability to form complexes with siRNA. Next, the characterisation of dendriplexes formed at different molar ratios was carried out using biophysical methods. The effects of zeta potential, size and changes of siRNA conformation on the complexation with dendrimers were examined. It was found that both phosphorus dendrimers interacted with siRNA. The zeta potential values of dendriplexes ranged from negative to positive and the hydrodynamic diameter depended on the number of dendrimer molecules in the complex. Furthermore, using circular dichroism spectroscopy it was found that cationic phosphorus dendrimers changed only slightly the shape of siRNA CD spectra, thus they did not induce significant changes in the nucleic acid secondary structure during complex formation.pl_PL
dc.description.sponsorshipStudies were funded by the project ―Biological properties and biomedical applications of dendrimers‖ operated within the Foundation for Polish Science Team Programme co-financed by the EU European Regional Development Fund. This work was also supported by the COST TD0802 project and by the CNRS, France.pl_PL
dc.language.isoenpl_PL
dc.publisherMDPIpl_PL
dc.relation.ispartofseriesMolecules;18
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectsiRNApl_PL
dc.subjectphosphorus dendrimerspl_PL
dc.subjectchronic myeloid leukemiapl_PL
dc.subjectgene therapypl_PL
dc.subjectdendriplexpl_PL
dc.titlePhosphorus Dendrimers as Carriers of siRNA—Characterisation of Dendriplexespl_PL
dc.typeArticlepl_PL
dc.page.number4451-4466pl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationDivision of Radiobiology, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Polandpl_PL
dc.contributor.authorAffiliationLaboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse Cedex 4, Francepl_PL
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dc.identifier.doi10.3390/molecules18044451
dc.relation.volume4pl_PL
dc.disciplinenauki biologicznepl_PL


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