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Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity

dc.contributor.authorŁojewska, Ewelina
dc.contributor.authorSakowicz, Tomasz
dc.contributor.authorKowalczyk, Aleksandra
dc.contributor.authorKonieczka, Magdalena
dc.contributor.authorGrzegorczyk, Janina
dc.contributor.authorSitarek, Przemysław
dc.contributor.authorSkała, Ewa
dc.contributor.authorCzarny, Piotr
dc.contributor.authorŚliwiński, Tomasz
dc.contributor.authorKowalczyk, Tomasz
dc.date.accessioned2021-10-15T09:48:35Z
dc.date.available2021-10-15T09:48:35Z
dc.date.issued2019
dc.identifier.citationŁojewska, E., Sakowicz, T., Kowalczyk, A. et al. Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity. Plant Biotechnol Rep 14, 33–43 (2020). https://doi.org/10.1007/s11816-019-00571-ypl_PL
dc.identifier.issn1863-5466
dc.identifier.urihttp://hdl.handle.net/11089/39395
dc.description.abstractAntibiotic-resistant microorganisms causing a life-threatening infection pose a serious challenge for modern science. The rapidly growing number of incidents for which the use of standard antibiotics is ineffective forces us to develop new alternative methods of killing microorganisms. Antimicrobial proteins and peptides (AMPs) can be promising candidates to solve this problem. Colicin-M is one of the representatives of this group and is naturally produced by Escherichia coli acting on other closely related bacterial strains by disrupting their outer cell membrane. This bacteriocin has huge potential as a potent antimicrobial agent, especially, since it was recognized by the FDA as safe. In this work, we present the expression of colicin M in model transgenic Nicotiana tabacum L. plants. We demonstrate that purified colicin retains its antibacterial activity against the control Escherichia coli strains and clinical isolates of Escherichia coli and Klebsiella pneumoniae. Our results also show that plant-derived ColM is not toxic for L929 and HeLa cell line, which allows us to suppose that plant-based expression could be an alternative production method of such important proteins.pl_PL
dc.language.isoenpl_PL
dc.publisherSpringer Naturepl_PL
dc.relation.ispartofseriesPlant Biotechnology Reports;14
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPlant-derived recombinant proteinpl_PL
dc.subjectPlant expression systemspl_PL
dc.subjectBacteriocinspl_PL
dc.subjectColicin Mpl_PL
dc.subjectNicotiana tabacumpl_PL
dc.titleProduction of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activitypl_PL
dc.typeArticlepl_PL
dc.page.number33–43pl_PL
dc.contributor.authorAffiliationDepartment of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbial Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha12/16, 90-237, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego 1, 90-151, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego 1, 90-151, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Medical Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215, Lodz, Polandpl_PL
dc.contributor.authorAffiliationLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Polandpl_PL
dc.identifier.eissn1863-5474
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dc.identifier.doi10.1007/s11816-019-00571-y
dc.relation.volume1pl_PL
dc.disciplinenauki biologicznepl_PL


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