Optimization of flotation assay conditions for syndapin binding to phosphatidic acid containing liposomes

Piaścik, Magda; Zegarlińska, Jolanta; Sikorski, Aleksander F.; Czogalla, Aleksander

dc.contributor.author	Piaścik, Magda	en
dc.contributor.author	Zegarlińska, Jolanta	en
dc.contributor.author	Sikorski, Aleksander F.	en
dc.contributor.author	Czogalla, Aleksander	en
dc.date.accessioned	2018-04-03T09:00:06Z
dc.date.available	2018-04-03T09:00:06Z
dc.date.issued	2017-12-29	en
dc.identifier.issn	1730-2366	en
dc.identifier.uri	http://hdl.handle.net/11089/24314
dc.description.abstract	Flotation is one of the best method for preliminary identification of protein-lipid interactions. In most widely used approach it utilizes large unilamellar vesicles, that are excellent models of freestanding membranes and do not require any additional components, like solid supports or beads that are needed in other methods commonly used for protein-lipid binding studies. Here we present results obtained during our studies on phosphatidic acid - syndapin interactions and discuss some technical aspects of this method underlying how relatively small changes in the conditions can influence the results.	en
dc.publisher	Wydawnictwo Uniwersytetu Łódzkiego	en
dc.relation.ispartofseries	Folia Biologica et Oecologica;13	en
dc.rights	This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0	en
dc.subject	protein-lipid interactions	en
dc.subject	LUVs	en
dc.subject	density gradient	en
dc.subject	ultracentrifugation	en
dc.title	Optimization of flotation assay conditions for syndapin binding to phosphatidic acid containing liposomes	en
dc.page.number	9-17	en
dc.contributor.authorAffiliation	Piaścik, Magda - Department of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, ul. F. Joliot-Curie 14a, 50-383 Wrocław, Poland	en
dc.contributor.authorAffiliation	Zegarlińska, Jolanta - Department of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, ul. F. Joliot-Curie 14a, 50-383 Wrocław, Poland	en
dc.contributor.authorAffiliation	Sikorski, Aleksander F. - Department of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, ul. F. Joliot-Curie 14a, 50-383 Wrocław, Poland	en
dc.contributor.authorAffiliation	Czogalla, Aleksander - Department of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, ul. F. Joliot-Curie 14a, 50-383 Wrocław, Poland	en
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dc.contributor.authorEmail	Czogalla, Aleksander - aleksander.czogalla@uwr.edu.pl	en
dc.identifier.doi	10.1515/fobio-2017-0002	en

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