dc.contributor.author | Borowczyk, Kamila | |
dc.contributor.author | Olejarz, Patrycja | |
dc.contributor.author | Chwatko, Grażyna | |
dc.contributor.author | Szylberg, Marcin | |
dc.contributor.author | Głowacki, Rafał | |
dc.date.accessioned | 2021-09-09T07:12:25Z | |
dc.date.available | 2021-09-09T07:12:25Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Borowczyk K, Olejarz P, Chwatko G, Szylberg M, Głowacki R. A Simplified Method for Simultaneous Determination of α-Lipoic Acid and Low-Molecular-Mass Thiols in Human Plasma. International Journal of Molecular Sciences. 2020; 21(3):1049. https://doi.org/10.3390/ijms21031049 | pl_PL |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/11089/39006 | |
dc.description.abstract | α-Lipoic acid, glutathione, cysteine, and cysteinylglycine can be applied as therapeutic
agents in civilization diseases such as diabetes mellitus, cardiovascular diseases, and cancers. On the
other hand, a higher concentration of homocysteine can result in health problems and has been
indicated as an independent risk factor for cardiovascular disease and accelerated atherosclerosis.
Here, the first simplified HPLC-UV assay that enables simultaneous determination of α-lipoic acid and
low-molecular-mass thiols in plasma, reduces the number of steps, shortens the total time of sample
preparation, and limits the amount of single-use polypropylene laboratory materials is described.
The assay is based on reversed-phase high performance liquid chromatography with UV detection
and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective
pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide. Linearity in
the detector responses for plasma samples were observed in ranges: 0.12–5.0 nmol mL−1
for α-lipoic
acid; 2.0–20.0 nmol mL−1
for glutathione, cysteinylglycine, and homocysteine; and 40.0–400.0 for
cysteine. The LODs for α-lipoic acid and low-molecular-mass thiols were 0.08 and 0.12 nmol mL−1
,
respectively, while LOQs were 0.12 and 0.16 nmol mL−1
, respectively. The usefulness of the proposed
method has been proven by its application to real samples. | pl_PL |
dc.language.iso | en | pl_PL |
dc.publisher | MDPI | pl_PL |
dc.relation.ispartofseries | International Journal of Molecular Sciences;21 | |
dc.rights | Uznanie autorstwa 4.0 Międzynarodowe | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | α-lipoic acid | pl_PL |
dc.subject | low-molecular-mass thiols | pl_PL |
dc.subject | simultaneous reduction and derivatization | pl_PL |
dc.subject | high performance liquid chromatography | pl_PL |
dc.subject | ultraviolet detection | pl_PL |
dc.title | A Simplified Method for Simultaneous Determination of α-Lipoic Acid and Low-Molecular-Mass Thiols in Human Plasma | pl_PL |
dc.type | Article | pl_PL |
dc.page.number | 15 | pl_PL |
dc.contributor.authorAffiliation | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Łódź, Poland | pl_PL |
dc.contributor.authorAffiliation | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Łódź, Poland | pl_PL |
dc.contributor.authorAffiliation | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Łódź, Poland | pl_PL |
dc.contributor.authorAffiliation | Rehabilitation Center “Kraszewski”, Kraszewskiego 7/9, 93-161 Łódź, Poland | pl_PL |
dc.contributor.authorAffiliation | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Łódź, Poland | pl_PL |
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dc.identifier.doi | https://doi.org/10.3390/ijms21031049 | |
dc.discipline | nauki chemiczne | pl_PL |