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The use of high-performance liquid chromatography with diode array detector for the determination of sulfide ions in human urine samples using pyrylium salts

dc.contributor.authorZakrzewski, Robert
dc.contributor.authorZgagacz, W.
dc.contributor.authorUrbaniak, Katarzyna
dc.contributor.authorChwatko, Grażyna
dc.contributor.authorNowicki, A.
dc.date.accessioned2021-08-25T10:58:27Z
dc.date.available2021-08-25T10:58:27Z
dc.date.issued2020
dc.identifier.issn1570-0232
dc.identifier.urihttp://hdl.handle.net/11089/38840
dc.description.abstractHydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts – (2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino)phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C18 column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2–150 μmol L−1 and 1–50 μmol L−1 for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers.pl_PL
dc.language.isoenpl_PL
dc.publisherElsevierpl_PL
dc.relation.ispartofseriesJournal of Chromatography B;1157
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectjon siarczkowypl_PL
dc.subjectsole piryliowepl_PL
dc.subjectwysokosprawna chromatografia cieczowa (HPLC)pl_PL
dc.subjectdetektor diodowy (DAD)pl_PL
dc.subjectmoczpl_PL
dc.subjectSulfide ionspl_PL
dc.subjectPyrylium saltspl_PL
dc.subjectHigh performance liquid chromatography with diode detector (DAD)pl_PL
dc.subjectHuman urine samplepl_PL
dc.titleThe use of high-performance liquid chromatography with diode array detector for the determination of sulfide ions in human urine samples using pyrylium saltspl_PL
dc.typeArticlepl_PL
dc.page.number7pl_PL
dc.contributor.authorAffiliationDepartment of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Polandpl_PL
dc.contributor.authorAffiliationDepartment of Organic and Applied Chemistry, Faculty of Chemistry, University of Lodz, Polandpl_PL
dc.identifier.eissn1873-376X
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dc.identifier.doi10.1016/j.jchromb.2020.122309
dc.disciplinenauki chemicznepl_PL


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