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Gene and protein expression of O-GlcNAc-cycling enzymes in human laryngeal cancer

dc.contributor.authorStarska, Katarzyna
dc.contributor.authorForma, Ewa
dc.contributor.authorBrzezińska-Błaszczyk, Ewa
dc.contributor.authorLewy-Trenda, Iwona
dc.contributor.authorBryś, Magdalena
dc.contributor.authorJóźwiak, Paweł
dc.contributor.authorKrześlak, Anna
dc.date.accessioned2016-04-05T11:33:55Z
dc.date.available2016-04-05T11:33:55Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/11089/17685
dc.description.abstractAberrant protein O-GlcNAcylation may contribute to the development and malignant behavior of many cancers. This modification is controlled by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAcase (OGA). The aim of this study was to determine the expression of O-GlcNAc cycling enzymes mRNA/protein and to investigate their relationship with clinicopathological parameters in laryngeal cancer. The mRNA levels of OGT and MGEA5 genes were determined in 106 squamous cell laryngeal cancer (SCLC) cases and 73 non-cancerous adjacent laryngeal mucosa (NCLM) controls using quantitative real-time PCR. The level of OGT and OGA proteins was analyzed by Western blot. A positive expression of OGT and MGEA5 transcripts and OGT and OGA proteins was confirmed in 75.5 and 68.9 % and in 43.7 and 59.4 % samples of SCLC, respectively. Higher levels of mRNA/protein for both OGT and OGA as well as significant increases of 60 % in total protein O-GlcNAcylation levels were noted in SCLC compared with NCLM (p < 0.05). As a result, an increased level of OGT and MGEA5 mRNA was related to larger tumor size, nodal metastases, higher grade and tumor behavior according to TFG scale, as well as incidence of disease recurrence (p < 0.05). An inverse association between OGT and MGEA5 transcripts was determined with regard to prognosis (p < 0.05). In addition, the highest OGT and OGA protein levels were observed in poorly differentiated tumors (p < 0.05). No correlations with other parameters were noted, but the results showed a trend of more advanced tumors to be more frequently OGT and OGA positive. The results suggest that increased O-GlcNAcylation may have an effect on tumor aggressiveness and prognosis in laryngeal cancer.pl_PL
dc.description.sponsorshipThis work was supported, in part, by a grant from the National Science Council, Poland (N403 043 32/2326), by the statutory fund of the Department of Cytobiochemistry, University of Łódź, Poland (506/811)pl_PL
dc.language.isoenpl_PL
dc.publisherSpringer International Publishingpl_PL
dc.relation.ispartofseriesClinical and Experimental Medicine;4
dc.rightsUznanie autorstwa 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/pl/*
dc.subjectO-GlcNAcpl_PL
dc.subjectOGTpl_PL
dc.subjectOGA (MGEA5)pl_PL
dc.subjectTumor front grading (TFG)pl_PL
dc.subjectLaryngeal cancerpl_PL
dc.titleGene and protein expression of O-GlcNAc-cycling enzymes in human laryngeal cancerpl_PL
dc.typeArticlepl_PL
dc.page.number455-468pl_PL
dc.contributor.authorAffiliationUniversity of Łódź, Department of Cytobiochemistrypl_PL
dc.contributor.authorAffiliationMedical University of Łódźpl_PL
dc.identifier.eissn1591-9528
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dc.contributor.authorEmailkatarzyna.starska@umed.lodz.plpl_PL
dc.identifier.doi10.1007/s10238-014-0318-1
dc.relation.volume15pl_PL


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Uznanie autorstwa 3.0 Polska
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