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dc.contributor.authorGóralski, Paweł
dc.contributor.authorRogalinska, Malgorzata
dc.contributor.authorBłoński, Jerzy Z.
dc.contributor.authorPytel, Edyta
dc.contributor.authorRobak, Tadeusz
dc.contributor.authorKiliańska, Zofia M.
dc.contributor.authorPiekarski, Henryk
dc.description.abstractChronic lymphocytic leukemia (CLL) is a heterogenous disease with an imbalance between apoptosis and cell proliferation. Therefore, the main goal in CLL therapy is to induce apoptosis and effectively support this process in transformed B lymphocytes. In the current study, we have compared differential scanning calorimetry (DSC) profiles of nuclei isolated from CLL cells and normal mononuclear cells exposed to cladribine or fludarabine combined with mafosfamide (CM; FM), and additionally to CM combined with monoclonal antibody—rituximab (RCM) for 48 h, as well as in culture medium only (controls). Under current study, the mononuclear cells from peripheral blood (PBMCs) of healthy individuals have been included. The obtained results have shown the presence of thermal transition at 95 ± 5 °C in most of nuclear preparations (92.2 %) isolated from blood of CLL patients. This thermal characteristic parameter was changed after drug exposure, however, to a different extent. These thermal changes were accompanied by the decrease of cell viability, an elevation of apoptosis rate and the changes in expression/proteolysis of poly(ADP-ribose)polymerase-1—main marker of apoptosis. Importantly, in DSC profiles of nuclear preparations of PBMCs from blood of healthy donors exposed to investigated drug combinations and control CLL cells, the lack of such changes was observed. Our results confirmed that DSC technique complemented with other biological approaches could be helpful in tailoring therapy for CLL patients.pl_PL
dc.description.sponsorshipResearch was sponsored by Grant from the Polish National Science Centre (No. 2011/01/B/NZ/0102); Results of presented study were partially presented in oral presentation on 2nd Central and Eastern European Conference on Thermal Analysis and Calorimetry in Vilnius, Lithuania, 2013pl_PL
dc.publisherSpringer Netherlandspl_PL
dc.relation.ispartofseriesJournal of Thermal Analysis and Calorimetry;2
dc.rightsUznanie autorstwa 3.0 Polska*
dc.subjectChronic lymphocytic leukemiapl_PL
dc.subjectDifferential scanning calorimetrypl_PL
dc.subjectAnti-leukemic tailored therapypl_PL
dc.subjectPurine analogspl_PL
dc.titleThe differences in thermal profiles between normal and leukemic cells exposed to anticancer drug evaluated by differential scanning calorimetrypl_PL
dc.contributor.authorAffiliationUniversity of Lodz, Department of Physical Chemistrypl_PL
dc.contributor.authorAffiliationUniversity of Lodz, Department of Cytobiochemistrypl_PL
dc.contributor.authorAffiliationMedical University of Lodz, Department of Hematologypl_PL
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Uznanie autorstwa 3.0 Polska
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