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RNase HI Is Essential for Survival of Mycobacterium smegmatis

dc.contributor.authorMinias, Alina E.
dc.contributor.authorBrzostek, Anna M.
dc.contributor.authorKorycka- Machala, Malgorzata
dc.contributor.authorDziadek, Bozena
dc.contributor.authorMinias, Piotr
dc.contributor.authorRajagopalan, Malini
dc.contributor.authorMadiraju, Murty
dc.contributor.authorDziadek, Jaroslaw
dc.date.accessioned2015-09-02T08:05:50Z
dc.date.available2015-09-02T08:05:50Z
dc.date.issued2015-05-12
dc.identifier.citationMinias AE, Brzostek AM, Korycka- Machala M, Dziadek B, Minias P, Rajagopalan M, et al. (2015) RNase HI Is Essential for Survival of Mycobacterium smegmatis. PLoS ONE 10(5): e0126260. doi:10.1371/journal.pone.0126260pl_PL
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11089/11627
dc.description.abstractRNases H are involved in the removal of RNA from RNA/DNA hybrids. Type I RNases H are thought to recognize and cleave the RNA/DNA duplex when at least four ribonucleotides are present. Here we investigated the importance of RNase H type I encoding genes for model organism Mycobacterium smegmatis. By performing gene replacement through homologous recombination, we demonstrate that each of the two presumable RNase H type I encoding genes, rnhA and MSMEG4305, can be removed from M. smegmatis genome without affecting the growth rate of the mutant. Further, we demonstrate that deletion of both RNases H type I encoding genes in M. smegmatis leads to synthetic lethality. Finally, we question the possibility of existence of RNase HI related alternative mode of initiation of DNA replication in M. smegmatis, the process initially discovered in Escherichia coli. We suspect that synthetic lethality of double mutant lacking RNases H type I is caused by formation of R-loops leading to collapse of replication forks. We report Mycobacterium smegmatis as the first bacterial species, where function of RNase H type I has been found essential.pl_PL
dc.description.sponsorshipThe study was supported by POIG.01.01.02-10-107/09 project implemented under Innovative Economy Operational Programme, years 2007–2013 "Studies of the molecular mechanisms at the interface the human organism - the pathogen - environmental factors" and by grant of Polish National Center of Science 2011/01/N/NZ6/04186 “Identification of a novel mechanism of initiation of DNA replication in Mycobacterium smegmatis”.pl_PL
dc.language.isoenpl_PL
dc.publisherPublic Library of Sciencepl_PL
dc.relation.ispartofseriesPLoS One;5
dc.rightsUznanie autorstwa 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/pl/*
dc.titleRNase HI Is Essential for Survival of Mycobacterium smegmatispl_PL
dc.typeArticlepl_PL
dc.rights.holder© 2015 Minias et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pl_PL
dc.page.number1-20pl_PL
dc.contributor.authorAffiliationMinias Alina E., Institute of Medical Biology, Polish Academy of Sciences, Lodzpl_PL
dc.contributor.authorAffiliationBrzostek Anna M., Institute of Medical Biology, Polish Academy of Sciences, Lodzpl_PL
dc.contributor.authorAffiliationKorycka- Machala Malgorzata,Institute of Medical Biology, Polish Academy of Sciences, Lodzpl_PL
dc.contributor.authorAffiliationDziadek Bozena, Department of Immunoparasitology, University of Lodzpl_PL
dc.contributor.authorAffiliationMinias Piotr, Department of Teacher Training and Biodiversity Studies, University of Lodzpl_PL
dc.contributor.authorAffiliationRajagopalan Malini, Department of Microbiology, University of Texas Health Center at Tyler, Tyler, Texaspl_PL
dc.contributor.authorAffiliationMadiraju Murty, Department of Microbiology, University of Texas Health Center at Tyler, Tyler, Texaspl_PL
dc.contributor.authorAffiliationDziadek Jaroslaw, Institute of Medical Biology, Polish Academy of Sciences, Lodzpl_PL
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dc.contributor.authorEmailalinagorna@gmail.plpl_PL
dc.contributor.authorEmailjdziadek@cbm.pan.plpl_PL
dc.identifier.doi10.1371/journal.pone.0126260
dc.relation.volume10pl_PL


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