http://hdl.handle.net/11089/1 2026-04-23T09:49:50Z http://hdl.handle.net/11089/58146 Charakterystyka kompleksów płytkowo-leukocytarnych i analiza ekspresji miRNA jako wskaźników stanu zapalnego i neurodegeneracji w rzutowo-remisyjnej i wtórnie postępującej postaci stwardnienia rozsianego Wasilewska, Karina Multiple sclerosis (MS) is a chronic neurodegenerative disease with an autoimmune background primarily affecting the central nervous system (CNS). The most common form of the condition is inflammatory relapsing-remitting MS (RRMS), whereas secondary progressive MS (SPMS), which typically develops after RRMS, is characterized by progressive neurodegeneration. However, recent reports indicate coexistence of inflammatory and neurodegenerative features in both phenotypes, complicating early differentiation, therapeutic decisions, and prognosis. The primary objective of the research was to identify markers enabling differentiation of disease phenotypes in patients with RRMS in remission and SPMS. In the first stage, platelet-leukocyte hetero-aggregates (PLAs) were characterized as elements linking vascular injury with inflammation. Increased leukocyte chemotaxis toward platelets and PLAs formation, predominantly involving B-cells, were demonstrated in MS. Next, screening of miRNA expression from extracellular vesicles (EVs) was conducted, followed by measurement of inflammatory cytokines and markers of neuronal/glial damage in plasma. A model combining miRNA expression with basic fibroblast growth factor achieved an AUC of 0.97, confirming its high ability to discriminate RRMS and SPMS. In the third stage, EVs presenting L1CAM, a protein used to enrich the neuronal fraction of EVs, were characterized in serum and cerebrospinal fluid to assess their usefulness as biomarkers for monitoring response to rituximab treatment, an anti-CD20 monoclonal antibody, in RRMS patients. In summary, the research expands understanding of MS pathophysiology, linking immune response with vascular injury, and identifies opportunities for non-invasive biomarkers to differentiate RRMS and SPMS as well as to monitor CNS immunopathology and treatment response. 2025-01-01T00:00:00Z http://hdl.handle.net/11089/58145 Aktywność biologiczna i toksyczność nanocząstek srebra pozyskanych na drodze mikrobiologicznej przy udziale grzyba strzępkowego Gloeophyllum striatum Tończyk, Aleksandra Silver nanoparticles (AgNPs) are among the most widely commercialized nanomaterials due to their versatile properties and strong antimicrobial activity, making them valuable in electronics, textiles, cosmetics, and biomedicine. The increasing demand for AgNPs highlights the need for synthesis methods that are cost-effective, rapid, and environmentally sustainable. Biological synthesis, particularly using filamentous fungi, offers an attractive alternative to conventional methods due to their ease of cultivation, high biomass production, and ability to produce metabolites influencing nanoparticle properties. This doctoral dissertation focused on the microbiological synthesis of silver nanoparticles using the brown-rot wood fungus Gloeophyllum striatum DSM 9592 under various process conditions, followed by evaluation of their antimicrobial, cytotoxic, ecotoxic, and synergistic activity with antibiotics. The results confirmed that G. striatum can efficiently synthesize silver nanoparticles, and that synthesis conditions, such as temperature and shaking, significantly affect their physicochemical and biological properties. The synthesized nanoparticles demonstrated antimicrobial activity against tested microorganisms, with fungi showing greater sensitivity than bacteria. The most sensitive organisms included the yeast Malassezia furfur and the bacterium Pseudomonas aeruginosa. Toxicity assessments revealed variable cytotoxic and ecotoxic effects depending on synthesis conditions. Nanoparticles synthesized at 4°C exhibited the lowest cytotoxicity while maintaining antimicrobial effectiveness. Ecotoxicity studies showed the highest sensitivity in the freshwater crustacean Daphnia magna and the lowest in tested crop plants. Overall, nanoparticles synthesized at 4°C without shaking showed the most favorable balance between antimicrobial activity and low toxicity. Additionally, these nanoparticles exhibited synergistic effects with antibiotics, enabling dose reduction. The findings demonstrate that optimization of fungal biosynthesis conditions enables the production of effective antimicrobial nanomaterials with reduced toxic potential. 2025-01-01T00:00:00Z http://hdl.handle.net/11089/57929 Wstępna ocena potencjału terapeutycznego miechunki pomidorowej (Physalis philadelphica) w kontekście przebudowy tkanek i gojenia ran (dataset) Liudvytska, Oleksandra Chronic wounds represent a growing problem in lifestyle diseases (e.g., atherosclerosis, diabetes). They are characterized, among other things, by disturbances in the mechanisms controlling the course of inflammatory processes, degeneration of the tissue surrounding the wound, scarring, and susceptibility to infections. Hard‑to‑heal wounds constitute a serious and burdensome issue for patients, as they significantly reduce their quality of life and require long-term, specialized therapy. The search for natural bioactive substances is one of the important directions in contemporary wound‑healing research. Natural compounds can serve as the basis for therapies that support all stages of healing, including modulation of the inflammatory response, fibroblast proliferation, and epithelial regeneration. They are often characterized by high biocompatibility and low cytotoxicity, making them very promising candidates for applications in wound‑healing support. The project focused on key aspects relevant to the potential use of extracts from the tomatillo (Physalis philadelphica) in wound‑healing support: anti‑inflammatory properties, effects on tissue remodeling, assessment of cytotoxicity risk, and metabolism by the skin microbiome. Four extracts were tested: from fruits (green – unripe, and yellow), leaves, and roots. The study employed dermal (fibroblast) and epithelial (keratinocyte) cell lines. Additionally, the effects of the extracts on proteins of the fibrinolytic system were evaluated, as this system plays an important regulatory role in tissue remodeling within the wound area. Spis plików: 1. Generowanie plazminy 2. IL- 6 3. IL- 8 4. Mikrobiota skóry 5. MMP3 6. MMP9 7. MTT 8. PLUA 9. PLUAR soluble 10. PLUAR 11. Test rany 2026-03-31T00:00:00Z http://hdl.handle.net/11089/57731 Opracowanie modelu badawczego in vitro do analiz wpływu stanu ketozy na angiogenezę nowotworową; Miniatura 8 (2024/08/X/NZ3/00599) (dataset) Soboska, Kamila As part of the Miniatura 8 research project, the effect of the ketosis state on tumour-associated angiogenesis was investigated. An in vitro model reflecting the conditions during ketogenic diet was developed, and the effects of glucose deprivation and ketone bodies were compared in tumour (TECs) and normal (NECs) endothelial cells. The studies were performed using the HMEC-1 cell line, in which a tumour endothelial cell model was generated under hypoxic conditions and in the presence of cancer cell-secreted pro-angiogenic factors. The expression of ketone body transporters and ketolytic enzymes was analysed at both the mRNA and protein levels in NECs and TECs, and their ability to metabolise ketone bodies was compared. In further experiments, the effect of ketone bodies on endothelial cell viability and the secretion of pro-angiogenic factors by colorectal cancer cell lines were evaluated. The dataset contains the results of the following tests: sqPCR from the analysis of the expression of markers confirming tumor endothelial cell model (TECs markers_sqPCR_RawData) and from the analysis of the expression of genes encoding ketolytic enzymes and the ketone body transporter (ketolysis_sqPCR_RawData), images of the results of the Western immunoblot analysis (WB scan), acetyl-CoA concentration in cells treated with ketone bodies (Acetyl-CoA conc_RawData), results of endothelial cell proliferation tests under conditions of variable glucose concentration (ECs prolif_glucose conc_RawData) and in conditions of simulated ketosis (ECs prolif_KBs conc_RawData) and results of the analysis of cytokine profiling secreted by tumor cells under simulated ketosis conditions (ProteomeProfiler) 2024-09-01T00:00:00Z