dc.contributor.author | Szewczyk, Rafał | |
dc.contributor.author | Soboń, Adrian | |
dc.contributor.author | Różalska, Sylwia | |
dc.contributor.author | Dzitko , Katarzyna | |
dc.contributor.author | Waidelich, Dietmar | |
dc.contributor.author | Długoński, Jerzy | |
dc.date.accessioned | 2015-06-15T09:23:24Z | |
dc.date.available | 2015-06-15T09:23:24Z | |
dc.date.issued | 2014-04-30 | |
dc.identifier.issn | 1879-0208 | |
dc.identifier.uri | http://hdl.handle.net/11089/9728 | |
dc.description.abstract | 4-n-nonylphenol (4-n-NP) is an endocrine disrupting compound (EDC); pollutants that cause serious
disturbances in the environment. This study shows the degradation pathway and initial proteome
analysis in cultures of a fungus that actively degrades 4-n-NP, Metarhizium robertsii. The research
revealed the presence of 14 4-n-NP metabolites formed as a result of the oxidation of the alkyl chain and
benzene ring, which leads to the complete decomposition of the compound. Based on the trend and
quantitative analysis of the formation of 4-n-NP derivatives, the best conditions for proteome analysis
were established. The data collected allowed the formulation of an explanation of the microorganism's
strategy towards the removal of 4-n-NP. The main groups of proteins engaged in the removal of the
xenobiotic are: oxidation-reduction systems related to nitroreductase-like proteins, ROS defense systems
(peroxiredoxin and superoxide dismutase), the TCA cycle and energy-related systems. Principal components
analysis was applied to unidentified proteins, resulting in the formulation of three subgroups
and initial classification of these proteins. | pl_PL |
dc.language.iso | en | pl_PL |
dc.publisher | Elsevier | pl_PL |
dc.relation.ispartofseries | International Biodeterioration & Biodegradation;Volume 93, September 2014 | |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska | * |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/pl/ | * |
dc.subject | Metarhizium robertsii | pl_PL |
dc.subject | fungi | pl_PL |
dc.subject | 4-Nonylphenol | pl_PL |
dc.subject | Biodegradation | pl_PL |
dc.subject | Proteomics | pl_PL |
dc.subject | Mass spectrometry | pl_PL |
dc.subject | lc-ms/ms | pl_PL |
dc.title | Intracellular proteome expression during 4-n-nonylphenol biodegradation by the filamentous fungus Metarhizium robertsii | pl_PL |
dc.type | Article | pl_PL |
dc.page.number | 44-53 | pl_PL |
dc.contributor.authorAffiliation | Faculty of Biology and Environmental Protection, University of Łodz, | pl_PL |
dc.contributor.authorAffiliation | AB SCIEX Germany GmbH | pl_PL |
dc.identifier.doi | 10.1016/j.ibiod.2014.04.026 | |