An Evaluation of the DNA-Protective Effects of Extracts from Menyanthes trifoliata L. Plants Derived from In Vitro Culture Associated with Redox Balance and Other Biological Activities
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2019-10-16Autor
Sitarek, Przemysław
Skała, Ewa
Rijo, Patrícia Dias de Mendonça
Andrade, Joana
Szemraj, Janusz
Krajewska, Urszula
Śliwiński, Tomasz
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Menyanthes trifoliata L. is a valuable medical plant found in Europe, North America, and Asia, which grows on peat bogs and
swamps. It has long been used in folk medicine as a remedy for various ailments. This is the first report to demonstrate the
protective antioxidant and anti-inflammatory properties of aqueous methanolic extracts derived from the aerial parts (MtAPV)
and roots (MtRV) of in vitro grown plants on human umbilical vein endothelial cells (HUVECs). It describes the influence of
the tested extracts on the expression of antioxidant (HO-1, NQO1, NRF2, kEAP1, and GCLC) and inflammation-related genes
(IL-1α, IL-1β, IL-6, TNF-α, and IFN-γ) in cells stimulated with H2O2 or LPS, respectively. In addition, M. trifoliata extracts were
found to moderately affect the growth of certain bacterial and fungal pathogens, with the strongest antibacterial effect found
against Pseudomonas aeruginosa and Enterococcus faecalis. M. trifoliata extracts demonstrated protective effects against
mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) damage caused by ROS, decreasing the numbers of mtDNA lesions
in the ND1 and ND2 genes and nDNA damage in the TP53 and HPRT1 genes and reducing cleavage in PARP1- and γ-H2A.Xpositive
cells. The root extract of in vitro M. trifoliata (MtRV) appears to have better anti-inflammatory, antioxidant,
antimicrobial, and protective properties than the extract from the aerial part (MtAPV). These differences in biological properties
may result from the higher content of selected phenolic compounds and betulinic acid in the MtRV than in the MtAPV extract.
1. Introduction
Health-promoting properties of plants have been used in the
prevention and therapy of many human diseases for thousands
of years. Currently, it is estimated that 300,000 plant
species exist worldwide [1]; however, relatively few have confirmed
therapeutic or protective properties. Fortunately,
modern methods and equipment allow much faster and
Hindawi
Oxidative Medicine and Cellular Longevity
Volume 2019, Article ID 9165784, 13 pages
https://doi.org/10.1155/2019/9165784
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