The Level of Isoprostanes as a Non-invasive Marker for in vivo Lipid Peroxidation in Secondary Progressive Multiple Sclerosis
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2011-03-12Autor
Miller, Elżbieta
Mrowicka, Małgorzata
Saluk-Juszczak, Joanna
Majsterek, Ireneusz
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Oxidative stress leads to lipid peroxidation and
may contribute to the pathogenesis of lesions in multiple
sclerosis (MS), an autoimmune disease characterized by
inflammatory as well as degenerative phenomena. Isoprostanes
are prostaglandin-like compounds which are
formed by free radical catalysed peroxidation of arachidonic
acid esterified in membrane phospholipids. They are
a new class of sensitive specific markers for in vivo lipid
peroxidation. In this study 26 patients (15 females and 11
males; mean age 48.2 ± 15.2 year; mean disease duration
10.0 ± 6.5 year) with secondary progressive MS (SPMS)
and 12 healthy controls were enrolled. In patients with
multiple sclerosis the lipid peroxidation as the level of
urine isoprostanes and the level of thiobarbituric acid
reactive species (TBARS) in plasma were estimated.
Moreover, we estimated the total antioxidative status
(TAS) in plasma. It was found that the urine isoprostanes
level was over 6-fold elevated in patients with SPMS than
in control (P\0.001). In SPMS patients TBARS level
was also statistically higher than in controls (P\0.01).However, we did not observed any difference of TAS level
in serum between SPMS patients and controls (P[0.05).
In patients with SPMS the lipid peroxidation and oxidative
stress measured as the increased level of isoprostanes was
observed. Thus, we suggest that the level of isoprostanes
may be used as non-invasive marker for a determination of
oxidative stress what in turn, together with clinical symptoms,
may determine an specific antioxidative therapy in
SPMS patients.
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