Badanie oddziaływania produktów degradacji Paraquatu i Gramoxonu z hemoglobiną człowieka i ryby
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In this work the effect of Paraquat (PQ) and Graraoxone (GX) on isolated human and carp hemoglobins was studied. Degradation products of PQ were isolated from GX solution by thin layer chromatography on silica gel platelets. Purity of the GX fractions obtained was verified on the basis of silica gel rechromatography. The fractions were identified by IR, UV and visible spectra. Binding of PQ degradation products to hemoglobin was studied by molecular filtration through a Sephadex G-50 column. Interaction of GX fractions with hemoglobin was studied by the spectrophotometric method. Absorption spectra were recorded in the X = 200-900 nm range in 30-min intervals, between 0 and 3 hrs of incubation. Effect of pH of the medium on the amount of hemoglobin-bound ligand was determined, and the reactivity of the GX fractions obtained was compared with those of PQ and GX. Conditions of the strongest and the weakest binding of the ligands were identified. The studies performed point to a considerable importance of Hb in the transport of PQ and its degradation products to various tissues. The stronger binding of these ligands by carp Hb confirms that fishes are more sensitive to poisoning by bipyridyl derivatives as compared with mammals (primates).