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dc.contributor.authorDyson, Rebecca M.
dc.contributor.authorPalliser, Hannah K.
dc.contributor.authorLatter, Joanna L.
dc.contributor.authorKelly, Megan A.
dc.contributor.authorChwatko, Grazyna
dc.contributor.authorGlowacki, Rafal
dc.contributor.authorWright, Ian M. R.
dc.description.abstractBackground & Aims Hydrogen sulphide (H2S), nitric oxide (NO), and carbon monoxide (CO) are involved in transitional microvascular tone dysregulation in the preterm infant; however there is conflicting evidence on the interaction of these gasotransmitters, and their overall contribution to the microcirculation in newborns is not known. The aim of this study was to measure the levels of all 3 gasotransmitters, characterise their interrelationships and elucidate their combined effects on microvascular blood flow. Methods 90 preterm neonates were studied at 24h postnatal age. Microvascular studies were performed by laser Doppler. Arterial COHb levels (a measure of CO) were determined through co-oximetry. NO was measured as nitrate and nitrite in urine. H2S was measured as thiosulphate by liquid chromatography. Relationships between levels of the gasotransmitters and microvascular blood flow were assessed through partial correlation controlling for the influence of gestational age. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow and derive a theoretical model of their interactions. Results No relationship was observed between NO and CO (p = 0.18, r = 0.18). A positive relationship between NO and H2S (p = 0.008, r = 0.28) and an inverse relationship between CO and H2S (p = 0.01, r = -0.33) exists. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow. The model with the best fit is presented. Conclusions The relationships between NO and H2S, and CO and H2S may be of importance in the preterm newborn, particularly as NO levels in males are associated with higher H2S levels and higher microvascular blood flow and CO in females appears to convey protection against vascular dysregulation. Here we present a theoretical model of these interactions and their overall effects on microvascular flow in the preterm newborn, upon which future mechanistic studies may be based.pl_PL
dc.description.sponsorshipThe authors would like to acknowledge the parents of the neonates enrolled in the 2CANS study for their participation, the staff of the Kaleidoscope Neonatal Intensive Care Unit at the John Hunter Children’s Hospital, and Kimberly-Clark Australia for providing the diapers used in this studypl_PL
dc.publisherPublic Library Of Sciencepl_PL
dc.relation.ispartofseriesPLOS ONE;
dc.rightsUznanie autorstwa 3.0 Polska*
dc.titleInteractions of the Gasotransmitters Contribute to Microvascular Tone (Dys)regulation in the Preterm Neonatepl_PL
dc.contributor.authorAffiliationDyson Rebecca M., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastle, Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongongpl_PL
dc.contributor.authorAffiliationPalliser Hannah K., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Biomedical Sciences and Pharmacy, University of Newcastlepl_PL
dc.contributor.authorAffiliationLatter Joanna L., Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastlepl_PL
dc.contributor.authorAffiliationKelly, Megan A. Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongongpl_PL
dc.contributor.authorAffiliationChwatko Grazyna, University of Lodz, Department of Environmental Chemistry, Faculty of Chemistrypl_PL
dc.contributor.authorAffiliationGlowacki, Rafal, University of Lodz, Department of Environmental Chemistry, Faculty of Chemistrypl_PL
dc.contributor.authorAffiliationWright, Ian M. R. Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, School of Medicine and Public Health, University of Newcastle, Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongong, Kaleidoscope Neonatal Intensive Care Unit, John Hunter Children’s Hospitapl_PL
dc.referencesDyson RM, Palliser HK, Latter JL, Chwatko G, Glowacki R, et al. (2014) A role for H2S in the microcirculation of newborns: the major metabolite of H2S (thiosulphate) is increased in preterm infants. PloS one 9: e105085. doi: 10.1371/journal.pone.0105085 PMID: 25121737pl_PL
dc.referencesKrediet TG, Valk L, Hempenius I, Egberts J, van Bel F (2002) Nitric oxide production and plasma cyclic guanosine monophosphate in premature infants with respiratory distress syndrome. Biol Neonate 82: 150–154. PMID: 12373064pl_PL
dc.referencesFarkas I, Maroti Z, Katona M, Endreffy E, Monostori P, et al. (2008) Increased heme oxygenase-1 expression in premature infants with respiratory distress syndrome. Eur J Pediatr 167: 1379–1383. doi: 10.1007/s00431-008-0673-6 PMID: 18301921pl_PL
dc.referencesStark MJ, Clifton VL, Wright IM (2009) Carbon monoxide is a significant mediator of cardiovascular status following preterm birth. Pediatrics 124: 277–284. doi: 10.1542/peds.2008-0877 PMID: 19564310pl_PL
dc.referencesChen K, Popel AS (2006) Theoretical analysis of biochemical pathways of nitric oxide release from vascular endothelial cells. Free radical biology & medicine 41: 668–680.pl_PL
dc.referencesKavdia M, Popel AS (2004) Contribution of nNOS- and eNOS-derived NO to microvascular smooth muscle NO exposure. Journal of applied physiology 97: 293–301. PMID: 15033959pl_PL
dc.referencesKnecht KR, Milam S, Wilkinson DA, Fedinec AL, Leffler CW (2010) Time-dependent action of carbon monoxide on the newborn cerebrovascular circulation. Am J Physiol Heart Circ Physiol 299: H70–H75. doi: 10.1152/ajpheart.00258.2010 PMID: 20435844pl_PL
dc.referencesMaines MD (1997) The heme oxygenase system: a regulator of second messenger gases. Annu Rev Pharmacol Toxicol 37: 517–554. PMID: 9131263pl_PL
dc.referencesWu L, Wang R (2005) Carbon monoxide: endogenous production, physiological functions, and pharmacological applications. Pharmacol Rev 57: 585–630. PMID: 16382109pl_PL
dc.referencesCarson RJ, Seyffarth G, Mian R, Maddock H (2004) Interactions Between Gasotransmitters. In: Wang R, editor. Signal Transduction and the Gasotransmitters: NO, CO, and H2S in Biology and Medicine. 2004 ed. Totowa, NJ: Humana Press Inc.pl_PL
dc.referencesForesti R, Hoque M, Bains S, Green CJ, Motterlini R (2003) Haem and nitric oxide: synergism in the modulation of the endothelial haem oxygenase-1 pathway. Biochem J 372: 381–390. PMID: 12622689pl_PL
dc.referencesLeffler CW, Nasjletti A, Johnson RA, Fedinec AL (2001) Contributions of prostacyclin and nitric oxide to carbon monoxide-induced cerebrovascular dilation in piglets. Am J Physiol Heart Circ Physiol 280: H1490–1495. PMID: 11247758pl_PL
dc.referencesLeffler CW, Fedinec AL, Parfenova H, Jaggar JH (2005) Permissive contributions of NO and prostacyclin in CO-induced cerebrovascular dilation in piglets. Am J Physiol Heart Circ Physiol 289: H432–438. PMID: 15708959pl_PL
dc.referencesLeffler CW, Balabanova L, Fedinec AL, Parfenova H (2005) Nitric oxide increases carbon monoxide production by piglet cerebral microvessels. Am J Physiol Heart Circ Physiol 289: H1442–1447. PMID: 15964921pl_PL
dc.referencesPong WW, Eldred WD (2009) Interactions of the gaseous neuromodulators nitric oxide, carbon monoxide, and hydrogen sulfide in the salamander retina. J Neurosci Res 87: 2356–2364. doi: 10.1002/jnr. 22042 PMID: 19267415pl_PL
dc.referencesDurante W, Kroll MH, Christodoulides N, Peyton KJ, Schafer AI (1997) Nitric oxide induces heme oxygenase- 1 gene expression and carbon monoxide production in vascular smooth muscle cells. Circ Res 80: 557–564. PMID: 9118487pl_PL
dc.referencesDatta PK, Lianos EA (1999) Nitric oxide induces heme oxygenase-1 gene expression in mesangial cells. Kidney Int 55: 1734–1739. PMID: 10231435pl_PL
dc.referencesBouton C, Demple B (2000) Nitric oxide-inducible expression of heme oxygenase-1 in human cells. Translation-independent stabilization of the mRNA and evidence for direct action of nitric oxide. J Biol Chem 275: 32688–32693. PMID: 11032845pl_PL
dc.referencesLiang M, Croatt AJ, Nath KA (2000) Mechanisms underlying induction of heme oxygenase-1 by nitric oxide in renal tubular epithelial cells. Am J Physiol Renal Physiol 279: F728–735. PMID: 10997923pl_PL
dc.referencesAlcaraz MJ, Habib A, Creminon C, Vicente AM, Lebret M, et al. (2001) Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor. Biochim Biophys Acta 1526: 13–16. PMID: 11287117pl_PL
dc.referencesWang R (2002) Two's company, three's a crowd: can H2S be the third endogenous gaseous transmitter? FASEB J 16: 1792–1798. PMID: 12409322pl_PL
dc.referencesMotterlini R, Foresti R, Intaglietta M, Winslow RM (1996) NO-mediated activation of heme oxygenase: endogenous cytoprotection against oxidative stress to endothelium. Am J Physiol 270: H107–114. PMID: 8769740pl_PL
dc.referencesDing Y, McCoubrey WK Jr, Maines MD (1999) Interaction of heme oxygenase-2 with nitric oxide donors. Is the oxygenase an intracellular 'sink' for NO? Eur J Biochem 264: 854–861. PMID: 10491133pl_PL
dc.referencesZhu XY, Liu SJ, Liu YJ, Wang S, Ni X (2010) Glucocorticoids suppress cystathionine gamma-lyase expression and H2S production in lipopolysaccharide-treated macrophages. Cell Mol Life Sci 67: 1119– 1132. doi: 10.1007/s00018-009-0250-9 PMID: 20063035pl_PL
dc.referencesZhao W, Zhang J, Lu Y, Wang R (2001) The vasorelaxant effect of H(2)S as a novel endogenous gaseous K(ATP) channel opener. EMBO J 20: 6008–6016. PMID: 11689441pl_PL
dc.referencesTaoka S, Ohja S, Shan X, Kruger WD, Banerjee R (1998) Evidence for heme-mediated redox regulation of human cystathionine beta-synthase activity. J Biol Chem 273: 25179–25184. PMID: 9737978pl_PL
dc.referencesTaoka S, Banerjee R (2001) Characterization of NO binding to human cystathionine beta-synthase: possible implications of the effects of CO and NO binding to the human enzyme. J Inorg Biochem 87: 245–251. PMID: 11744062pl_PL
dc.referencesThom SR, Xu YA, Ischiropoulos H (1997) Vascular endothelial cells generate peroxynitrite in response to carbon monoxide exposure. Chem Res Toxicol 10: 1023–1031. PMID: 9305585pl_PL
dc.referencesThorup C, Jones CL, Gross SS, Moore LC, Goligorsky MS (1999) Carbon monoxide induces vasodilation and nitric oxide release but suppresses endothelial NOS. Am J Physiol 277: F882–889. PMID: 10600935pl_PL
dc.referencesIngi T, Cheng J, Ronnett GV (1996) Carbon monoxide: an endogenous modulator of the nitric oxide-cyclic GMP signaling system. Neuron 16: 835–842. PMID: 8608001pl_PL
dc.referencesWhite KA, Marletta MA (1992) Nitric oxide synthase is a cytochrome P-450 type hemoprotein. Biochemistry 31: 6627–6631. PMID: 1379068pl_PL
dc.referencesSheng WS, Hu S, Nettles AR, Lokensgard JR, Vercellotti GM, et al. (2010) Hemin inhibits NO production by IL-1beta-stimulated human astrocytes through induction of heme oxygenase-1 and reduction of p38 MAPK activation. J Neuroinflammation 7: 51. doi: 10.1186/1742-2094-7-51 PMID: 20822529pl_PL
dc.referencesMcMillan K, Bredt DS, Hirsch DJ, Snyder SH, Clark JE, et al. (1992) Cloned, expressed rat cerebellar nitric oxide synthase contains stoichiometric amounts of heme, which binds carbon monoxide. Proc Natl Acad Sci U S A 89: 11141–11145. PMID: 1280819pl_PL
dc.referencesMorikawa T, Kajimura M, Nakamura T, Hishiki T, Nakanishi T, et al. (2012) Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway. Proc Natl Acad Sci U S A 109: 1293–1298. doi: 10.1073/pnas.1119658109 PMID: 22232681pl_PL
dc.referencesJin HF, Du JB, Li XH, Wang YF, Liang YF, et al. (2006) Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells. Acta Pharmacol Sin 27: 1561–1566. PMID: 17112409pl_PL
dc.referencesPeng YJ, Nanduri J, Raghuraman G, Souvannakitti D, Gadalla MM, et al. (2010) H2S mediates O2 sensing in the carotid body. Proc Natl Acad Sci U S A 107: 10719–10724. PMID: 20556885pl_PL
dc.referencesOndrias K, Stasko A, Cacanyiova S, Sulova Z, Krizanova O, et al. (2008) H(2)S and HS(-) donor NaHS releases nitric oxide from nitrosothiols, metal nitrosyl complex, brain homogenate and murine L1210 leukaemia cells. Pflugers Arch 457: 271–279. doi: 10.1007/s00424-008-0519-0 PMID: 18458940pl_PL
dc.referencesHosoki R, Matsuki N, Kimura H (1997) The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem Biophys Res Commun 237: 527–531. PMID: 9299397pl_PL
dc.referencesTeague B, Asiedu S, Moore PK (2002) The smooth muscle relaxant effect of hydrogen sulphide in vitro: evidence for a physiological role to control intestinal contractility. Br J Pharmacol 137: 139–145. PMID: 12208769pl_PL
dc.referencesZhao W, Wang R (2002) H(2)S-induced vasorelaxation and underlying cellular and molecular mechanisms. Am J Physiol Heart Circ Physiol 283: H474–480. PMID: 12124191pl_PL
dc.referencesAli MY, Ping CY, Mok YY, Ling L, Whiteman M, et al. (2006) Regulation of vascular nitric oxide in vitro and in vivo; a new role for endogenous hydrogen sulphide? Br J Pharmacol 149: 625–634. PMID: 17016507pl_PL
dc.referencesOh GS, Pae HO, Lee BS, Kim BN, Kim JM, et al. (2006) Hydrogen sulfide inhibits nitric oxide production and nuclear factor-kappaB via heme oxygenase-1 expression in RAW264.7 macrophages stimulated with lipopolysaccharide. Free Radic Biol Med 41: 106–119. PMID: 16781459pl_PL
dc.referencesKubo S, Doe I, Kurokawa Y, Nishikawa H, Kawabata A (2007) Direct inhibition of endothelial nitric oxide synthase by hydrogen sulfide: contribution to dual modulation of vascular tension. Toxicology 232: 138–146. PMID: 17276573pl_PL
dc.referencesKubo S, Kurokawa Y, Doe I, Masuko T, Sekiguchi F, et al. (2007) Hydrogen sulfide inhibits activity of three isoforms of recombinant nitric oxide synthase. Toxicology 241: 92–97. PMID: 17888559pl_PL
dc.referencesQingyou Z, Junbao D, Weijin Z, Hui Y, Chaoshu T, et al. (2004) Impact of hydrogen sulfide on carbon monoxide/heme oxygenase pathway in the pathogenesis of hypoxic pulmonary hypertension. Biochem Biophys Res Commun 317: 30–37. PMID: 15047144pl_PL
dc.referencesDyson RM, Palliser HK, Lakkundi A, De Waal K, Clifton VL, et al. (2014) Early microvascular changes in the preterm neonate: a comparative study of the human and guinea pig. Physiol Rep 2: e12145. doi: 10.14814/phy2.12145 PMID: 25350751pl_PL
dc.referencesParry G, Tucker J, Tarnow-Mordi W, UK Neonatal Staffing Study Collaborative Group (2003) CRIB II: an update of the clinical risk index for babies score. Lancet 24: 1789–1791pl_PL
dc.referencesStark MJ, Hodyl NA, Wright IM, Clifton V (2011) The influence of sex and antenatal betamethasone exposure on vasoconstrictors and the preterm microvasculature. The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 24: 1215–1220pl_PL
dc.referencesAmey M, Butchard N, Hanson L, Kinross D, Mannion M, et al. (2008) Cautionary tales from the neonatal intensive care unit: diapers may mislead urinary output estimation in extremely low birthweight infants. Pediatr Crit Care Med 9: 76–79. doi: 10.1097/01.PCC.0000298550.29453.7D PMID: 18477917pl_PL
dc.referencesChwatko G, Bald E (2009) Determination of thiosulfate in human urine by high performance liquid chromatography. Talanta 79: 229–234. doi: 10.1016/j.talanta.2009.03.040 PMID: 19559870pl_PL
dc.referencesBentler PM, Stein JA (1992) Structural equation models in medical research. Stat Methods Med Res 1: 159–181. PMID: 1341656pl_PL
dc.referencesBeran TN, Violato C (2010) Structural equation modeling in medical research: a primer. BMC Res Notes 3: 267. doi: 10.1186/1756-0500-3-267 PMID: 20969789pl_PL
dc.referencesHu LT, Bentler PM (1999) Cutoff criteria for Fit Indexes in Covariance Structure Analysis: conventional criteria versus new alternatives. Structural Equation Modeling 6: 1–55.pl_PL
dc.referencesMacCallum RC, Browne MW, Sugawara HM (1996) Power analysis and determination of sample size for Covariance Structure Modeling. Psychological Methods 1: 130–149pl_PL
dc.referencesAltaany Z, Ju Y, Yang G, Wang R (2014) The coordination of S-sulfhydration, S-nitrosylation, and phosphorylation of endothelial nitric oxide synthase by hydrogen sulfide. Science signaling 7: ra87. doi: 10. 1126/scisignal.2005478 PMID: 25205851pl_PL
dc.referencesDyson RM, Palliser HK, Ni X, Wright IMR (2014) Cystathionine-γ-lyase: a potential target for treatment of microvascular dysregulation following preterm birth? (PS334). Journal of Pediatrics and Child Health 50 (S1): 40–64pl_PL
dc.referencesApplegarth DA, Hardwick DF, Ross PM (1968) Creatinine excretion in children and the usefulness of creatinine equivalents in amino acid chromatography. Clin Chim Acta 22: 131–134. PMID: 5687081pl_PL
dc.referencesApplegarth DA, Ross PM (1975) The unsuitability of creatinine excretion as a basis for assessing the excretion of other metabolites by infants and children. Clin Chim Acta 64: 83–85. PMID: 1183029pl_PL
dc.referencesPolglase GR, Hooper SB, Kluckow M, Gill AW, Harding R, et al. (2012) The cardiopulmonary haemodynamic transition at birth is not different between male and female preterm lambs. Reproduction, fertility, and development 24: 510–516. doi: 10.1071/RD11121 PMID: 22401283pl_PL
dc.referencesEiby YA, Wright LL, Kalanjati VP, Miller SM, Bjorkman ST, et al. (2013) A pig model of the preterm neonate: anthropometric and physiological characteristics. PloS one 8: e68763. doi: 10.1371/journal.pone. 0068763 PMID: 23874755pl_PL
dc.referencesGrunfeld JP (1990) Glucocorticoids in blood pressure regulation. Horm Res 34: 111–113. PMID: 2104395pl_PL
dc.referencesBaum M, Moe OW (2008) Glucocorticoid-mediated hypertension: does vascular smooth muscle hold all the ansmwers? JASN 19: 1251–1253. doi: 10.1681/ASN.2008040410 PMID: 18508960pl_PL
dc.referencesWallerath T, Witte K, Schafer SC, Schwarz PM, Prellwitz W, et al. (1999) Down-regulation of the expression of endothelial NO synthase is likely to contribute to glucocorticoid-mediated hypertension. Proc Natl Acad Sci U S A 96: 13357–13362. PMID: 10557325pl_PL
dc.referencesWhitworth JA, Schyvens CG, Zhang Y, Andrews MC, Mangos GJ, et al. (2002) The nitric oxide system in glucocorticoid-induced hypertension. J Hypertens 20: 1035–1043. PMID: 12023661pl_PL
dc.referencesWen C, Li M, Whitworth JA (2000) Role of nitric oxide in adrenocorticotrophin-induced hypertension: Larginine effects reversed by N-nitro-L-arginine. Clin Exp Pharmacol Physiol 27: 887–890. PMID: 11071304pl_PL
dc.referencesWallerath T, Godecke A, Molojavyi A, Li H, Schrader J, et al. (2004) Dexamethasone lacks effect on blood pressure in mice with a disrupted endothelial NO synthase gene. Nitric Oxide 10: 36–41. PMID: 15050533pl_PL
dc.referencesKleinert H, Euchenhofer C, Ihrig-Biedert I, Forstermann U (1996) Glucocorticoids inhibit the induction of nitric oxide synthase II by down-regulating cytokine-induced activity of transcription factor nuclear factor- kappa B. Mol Pharmacol 49: 15–21. PMID: 8569701pl_PL
dc.referencesSimmons WW, Ungureanu-Longrois D, Smith GK, Smith TW, Kelly RA (1996) Glucocorticoids regulate inducible nitric oxide synthase by inhibiting tetrahydrobiopterin synthesis and L-arginine transport. J Biol Chem 271: 23928–23937. PMID: 8798625pl_PL
dc.referencesSingh K, Balligand JL, Fischer TA, Smith TW, Kelly RA (1995) Glucocorticoids increase osteopontin expression in cardiac myocytes and microvascular endothelial cells. Role in regulation of inducible nitric oxide synthase. J Biol Chem 270: 28471–28478. PMID: 7499354pl_PL
dc.referencesBlecharz KG, Burck M, Bauersachs J, Thum T, Tsikas D, et al. (2014) Inhibition of proteosome-mediated glucocorticoid recepter degredation restores nitric oxide bioavailability in myocardial endothelial cells in vitro. Biology of the Cell Accepted manuscript online, 18 Apr 2014.pl_PL
dc.referencesSoriano RN, Ravanelli MI, Batalhao ME, Carnio EC, Branco LGS (2013) Glucocorticoids downregulate systemic nitric oxide synthesis and counteract overexpression of hepatic heme oxygenase-1 during endotoxin tolerance. CJPP 91: 861–865. doi: 10.1139/cjpp-2013-0028 PMID: 24144058pl_PL
dc.referencesUno S, Guo DF, Nakajima M, Ohi H, Imada T, et al. (1994) Glucocorticoid induction of rat angiotensin II type 1A receptor gene promoter. Biochem Biophys Res Commun 204: 210–215. PMID: 7945361pl_PL
dc.referencesSato A, Suzuki H, Nakazato Y, Shibata H, Inagami T, et al. (1994) Increased expression of vascular angiotensin II type 1A receptor gene in glucocorticoid-induced hypertension. J Hypertens 12: 511–516. PMID: 7930550pl_PL
dc.referencesHanda M, Kondo K, Suzuki H, Saruta T (1984) Dexamethasone hypertension in rats: role of prostaglandins and pressor sensitivity to norepinephrine. Hypertension 6: 236–241. PMID: 6373587pl_PL
dc.referencesSaruta T (1996) Mechanism of glucocorticoid-induced hypertension. Hypertens Res 19: 1–8. PMID: 8829818pl_PL
dc.referencesStark MJ, Wright IM, Clifton VL (2009) Sex-specific alterations in placental 11beta-hydroxysteroid dehydrogenase 2 activity and early postnatal clinical course following antenatal betamethasone. American journal of physiology Regulatory, integrative and comparative physiology 297: R510–514. doi: 10. 1152/ajpregu.00175.2009 PMID: 19535674pl_PL
dc.referencesWang R (2014) Gasotransmitters: growing pains and joys. Trends in biochemical sciences 39: 227– 232. doi: 10.1016/j.tibs.2014.03.003 PMID: 24767680pl_PL

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